Protein_Domain

Part:BBa_K165006:Design

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-26)

Zif268-HIV DNA-binding domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 73
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

[http://2010.igem.org/Team:Slovenia Team Slovenia 2010] deposited a modified variant of HivC intended for easier isolation, purification and other experiments. We added HIS tag to C-terminal side of HivC sequence and C-terminal split CFP on N-terminal part of HivC after an extension linker. We deposited modified variant of HivC as cCFP_link_HivC BBa_K323071 and additionally characterized it with several methods.

Source

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References

Reynolds L., Ullman C., Moore M., Isalan M., West M.J., Clapham P., Klug A., Choo Y. 2003. Repression of the HIV-1 5LTR promoter and inhibition of HIV-1 replication by using engineered zinc-finger transcription factors. PNAS 2003 (100) 4:1615–1620